Test of solution of fluorescein

Goal

Check the absorbance and fluorescence of a solution of fluorescein in order to find the lowest concentration required to have a high fluorescence.

Results

Spectro 1

Concentration [µg/L]	E	R	G	B	UV
14	0.25	0.008	0.298	1.743	13977
7	0.47	0.012	0.213	1.642	35406
1.4	0.68	0.005	0.088	1.036	66273
0.7	0.89	0.030	0.053	0.630	55499
0.35	1.11	-0.001	0.030	0.340	37857
0.14	1.41	0.113	0.195	0.164	18380
0.07	1.63	0.007	0.014	0.073	10000

Spectro 2

Concentration [µg/L]	E	R	G	B	UV
14	0.24	0.005	0.294	1.738	15022
7	0.48	0.011	0.208	1.641	35132
1.4	0.78	0.003	0.085	1.038	69277
0.7	1.02	0.027	0.048	0.631	56721
0.35	1.26	-0.002	0.025	0.339	38510
0.14	1.51	0.109	0.172	0.166	18355
0.07	1.77	0.007	0.018	0.081	10121
0.014	2.03	0.003	-0.006	0.014	2019
0.0014	2.27	-0.006	-0.014	0.003	110
0	6.71	0.004	0.002	0.000	16
0.0014	6.96	-0.001	-0.013	0.007	110
0	7.18	0.011	0.012	0.008	0
0.0014	7.39	-0.006	-0.015	0.002	108
0	7.63	0.009	0.010	0.007	11
0.0014	7.86	-0.008	-0.015	0.001	105

All data

Conclusions

We are able to detect 1.4µg per liter of fluorescein.